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ITC datum

Protein and Ligand
Protein name DNA Gyrase B N-Terminal 24 Kda Fragment (variant 2)
Ligand name Novobiocin Novobiocin diagram
Novobiocin
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 1.0 ± 0.1  
ΔG -41.0 ± 0.42 kJ/mol
ΔH -64.85 ± 0.42 kJ/mol
-TΔS -23.43 ± 0.42 kJ/mol
KD 0.09 ± 1.0 uM
ΔCP -1.23 ± kJ/mol/K
Notes on error determination The standard error for some entries has been estimated where the values from the fitting procedure are unreasonably small.
General comments
Warnings DeltaG (-41.00) != DeltaH (-64.85) - T.DeltaS (-23.43) (=-41.42). Tolerance range from -41.41 to -40.59
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 305.0 pH 7.4
Buffer Phosphate Buffer 20.00 mM
Cell content Protein
Reference Biochemistry [1997] 36, 9663-9673