ITC

Browse


ISMB logo
Biochemical Journal logo

ITC datum

Protein and Ligand
Protein name DNA Gyrase B N-Terminal 24 Kda Fragment (R136H)
Ligand name Novobiocin Novobiocin diagram
Novobiocin
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 1.0 ± 0.1  
ΔG -32.22 ± 0.42 kJ/mol
ΔH -89.96 ± 1.67 kJ/mol
-TΔS -57.74 ± 1.67 kJ/mol
KD 3.57 ± 50.0 uM
ΔCP -1.9 ± kJ/mol/K
Notes on error determination The standard error for some entries has been estimated where the values from the fitting procedure are unreasonably small.
General comments
Warnings
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 310.0 pH 7.4
Buffer Phosphate Buffer 20.00 mM
Cell content Protein
Reference Biochemistry [1997] 36, 9663-9673