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ITC datum

Protein and Ligand
Protein name DNA Gyrase B N-Terminal 24 Kda Fragment (variant 2)
Ligand name Novobiocin Novobiocin diagram
Novobiocin
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 0.9 ±  
ΔG -53.6 ± kJ/mol
ΔH -48.12 ± kJ/mol
-TΔS 5.49 ± kJ/mol
KD 0.03 ± uM
ΔCP ±
Notes on error determination Not applicable
General comments
Warnings DeltaG (-53.60) != -R.T.ln(Ka) (-42.70). Tolerance range from -54.14 to -53.07
Instrument Omega ITC Microcalorimeter
Temp. (K) 298.15 pH 7.5
Buffer EDTA 1.00 mM Tris HCl 50.00 mM Potassium Chloride 100.00 mM
Cell content Protein
Reference J. Am. Chem. Soc. [1996] 35, 5083-5092