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ITC datum

Protein and Ligand
Protein name DNA Gyrase B N-Terminal 24 Kda Fragment (variant 2)
Ligand name Dihydronovobiocin Dihydronovobiocin diagram
Dihydronovobiocin
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 0.9 ±  
ΔG -18.94 ± kJ/mol
ΔH -30.54 ± kJ/mol
-TΔS -11.6 ± kJ/mol
KD 0.04 ± uM
ΔCP ±
Notes on error determination Not applicable
General comments
Warnings DeltaG (-18.94) != -R.T.ln(Ka) (-42.23). Tolerance range from -19.13 to -18.75
DeltaG (-18.94) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow.
Instrument Omega ITC Microcalorimeter
Temp. (K) 298.15 pH 7.5
Buffer EDTA 1.00 mM Tris HCl 50.00 mM Potassium Chloride 100.00 mM
Cell content Protein
Reference J. Am. Chem. Soc. [1996] 35, 5083-5092