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ITC datum

Protein and Ligand
Protein name Alpha-Thrombin
Ligand name [((1r)-2-{(2s)-2-[({4-[amino(imino)methyl]benzyl}amino)carbonyl]azetidinyl}-1-cyclohexyl-2-oxoethyl)amino]acetic acid [((1r)-2-{(2s)-2-[({4-[amino(imino)methyl]benzyl}amino)carbonyl]azetidinyl}-1-cyclohexyl-2-oxoethyl)amino]acetic acid diagram
[((1r)-2-{(2s)-2-[({4-[amino(imino)methyl]benzyl}amino)carbonyl]azetidinyl}-1-cyclohexyl-2-oxoethyl)amino]acetic acid
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -47.9 ± kJ/mol
ΔH -37.0 ± 1.2 kJ/mol
-TΔS 10.9 ± kJ/mol
KD 0.0 ± uM
ΔCP ±
Notes on error determination Not available
General comments
Warnings
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.15 pH 7.8
Buffer Buffer Substance (Tris, Tris(hydroxymethyl)amino-methane; Tricine; N-tris(hydroxymethyl)methylglycine; Hepes; N-2-hydroxylethylpiperazine-N-2-ethanesulphonic acid; pyrophosphate) 50.00 mM PEG 8000 0.10 % Sodium Chloride Rubidium Chloride 100.00 mM
Cell content Protein
Reference J Mol Biol. [2001] 313, 593-614