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ITC datum

Protein and Ligand
Protein name Beta-Glucosidase
Ligand name 1-deoxynojirimycin 1-deoxynojirimycin diagram
1-deoxynojirimycin
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 0.37 ± 0.02  
ΔG -26.53 ± 0.08 kJ/mol
ΔH -36.36 ± 1.63 kJ/mol
-TΔS -9.83 ± 1.59 kJ/mol
KD 35.71 ± 1000.0 uM
ΔCP -0.51 ± 0.08 kJ/mol/K
Notes on error determination Not applicable
General comments
Warnings DeltaG (-26.53) != -R.T.ln(Ka) (-26.24). Tolerance range from -26.79 to -26.26
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 308.15 pH 7.0
Buffer Potassium Phosphate 100.00 mM
Cell content Protein
Reference J. Am. Chem. Soc. [2003] 125, 14313-14323