ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | DNA Gyrase B N-Terminal 24 Kda Fragment (variant 2) | ||
| Ligand name |
Novobiocin
 Novobiocin |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.0 | ± 0.1 | |
| ΔG | -41.0 | ± 0.42 | kJ/mol |
| ΔH | -71.13 | ± 0.42 | kJ/mol |
| -TΔS | -30.54 | ± 0.84 | kJ/mol |
| KD | 0.13 | ± 0.01 | uM |
| ΔCP | -1.23 | ± | kJ/mol/K |
| Notes on error determination |
The standard error for some entries has been estimated where the values from the fitting procedure are unreasonably small. |
General comments | |
| Warnings |
DeltaG (-41.00) != DeltaH (-71.13) - T.DeltaS (-30.54) (=-40.58). Tolerance range from -41.41 to -40.59 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 310.0 | pH | 7.4 |
| Buffer | Phosphate Buffer 20.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1997] 36, 9663-9673 | ||