ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | DNA Gyrase B N-Terminal 24 Kda Fragment (R136H) | ||
| Ligand name |
Novobiocin
 Novobiocin |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.9 | ± 0.1 | |
| ΔG | -33.89 | ± 0.42 | kJ/mol |
| ΔH | -59.83 | ± 0.42 | kJ/mol |
| -TΔS | -25.52 | ± 0.42 | kJ/mol |
| KD | 1.2 | ± 33.33 | uM |
| ΔCP | -1.9 | ± | kJ/mol/K |
| Notes on error determination |
The standard error for some entries has been estimated where the values from the fitting procedure are unreasonably small. |
General comments | |
| Warnings |
DeltaG (-33.89) != DeltaH (-59.83) - T.DeltaS (-25.52) (=-34.31). Tolerance range from -34.23 to -33.55 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 300.0 | pH | 7.4 |
| Buffer | Tris HCl 69.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1997] 36, 9663-9673 | ||