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ITC datum

Protein and Ligand
Protein name DNA Gyrase B N-Terminal 43 Kda Fragment
Ligand name Novobiocin Novobiocin diagram
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 0.9 ±  
ΔG -63.53 ± kJ/mol
ΔH -53.56 ± kJ/mol
-TΔS 9.98 ± kJ/mol
KD 0.02 ± uM
Notes on error determination Not applicable
General comments
Warnings DeltaG (-63.53) != -R.T.ln(Ka) (-43.60). Tolerance range from -64.17 to -62.90
DeltaG (-63.53) less than -60.00. It is difficult to interpred ITC data if DeltaG is less than -60.00 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments.
Instrument Omega ITC Microcalorimeter
Temp. (K) 298.15 pH 7.5
Buffer EDTA 1.00 mM Tris HCl 50.00 mM Potassium Chloride 100.00 mM
Cell content Protein
Reference J. Am. Chem. Soc. [1996] 35, 5083-5092