ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | DNA Gyrase B N-Terminal 43 Kda Fragment | ||
| Ligand name |
Novobiocin
 Novobiocin |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.9 | ± | |
| ΔG | -63.53 | ± | kJ/mol |
| ΔH | -53.56 | ± | kJ/mol |
| -TΔS | 9.98 | ± | kJ/mol |
| KD | 0.02 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaG (-63.53) != -R.T.ln(Ka) (-43.60). Tolerance range from -64.17 to -62.90 DeltaG (-63.53) less than -60.00. It is difficult to interpred ITC data if DeltaG is less than -60.00 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 7.5 |
| Buffer | EDTA 1.00 mM Tris HCl 50.00 mM Potassium Chloride 100.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Am. Chem. Soc. [1996] 35, 5083-5092 | ||