ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Lysozyme | ||
| Ligand name |
Chitobiose
 Chitobiose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.97 | ± 0.0 | |
| ΔG | -19.92 | ± 0.08 | kJ/mol |
| ΔH | -50.25 | ± 0.59 | kJ/mol |
| -TΔS | -30.33 | ± | kJ/mol |
| KD | 420.85 | ± | uM |
| ΔCP | -0.35 | ± 0.02 | kJ/mol/K |
| Notes on error determination |
Uncertainties represent standard deviation from regression analysis. |
General comments | |
| Warnings |
Affinity (420.8497) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-19.92) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow. |
||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 308.15 | pH | 4.7 |
| Buffer | Acetate 0.10 M | ||
| Cell content | Protein | ||
| Reference | Protein Sci. [2003] 12, 135-142 | ||