ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | Lysozyme | ||
Ligand name |
Chitotriose
Chitotriose |
||
Interaction type | Biological | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | 0.97 | ± 0.01 | |
ΔG | -28.53 | ± 0.04 | kJ/mol |
ΔH | -63.6 | ± 0.42 | kJ/mol |
-TΔS | -35.06 | ± | kJ/mol |
KD | 14.56 | ± | uM |
ΔCP | -0.5 | ± 0.01 | kJ/mol/K |
Notes on error determination |
Uncertainties represent standard deviation from regression analysis. |
General comments | |
Warnings | |||
Instrument | VP-ITC Microcalorimeter | ||
Temp. (K) | 308.15 | pH | 4.7 |
Buffer | Acetate 0.10 M | ||
Cell content | Protein | ||
Reference | Protein Sci. [2003] 12, 135-142 |