ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Trypsin | ||
| Ligand name |
Benzamidine-para-amide
 Benzamidine-para-amide |
||
| Interaction type | Misc | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -29.1 | ± | kJ/mol |
| ΔH | -30.2 | ± | kJ/mol |
| -TΔS | -1.4 | ± | kJ/mol |
| KD | 9.71 | ± | uM |
| ΔCP | -0.52 | ± | kJ/mol/K |
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
Table 1 (row 7) ambigous typo/error:
29.1 != -30.2 + +1.4
Difference: -0.3
Authors contacted. DeltaG (-29.10) != DeltaH (-30.20) - T.DeltaS (-1.40) (=-28.80). Tolerance range from -29.39 to -28.81 |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 303.15 | pH | 8.0 |
| Buffer | Tris 50.00 mM Calcium Chloride 10.00 mM | ||
| Cell content | Protein | ||
| Reference | Eur. J. Biochem. [2001] 268, 1554-1560 | ||