ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Disabled Homolog 1 (H81A) | ||
| Ligand name |
D-myo-inositol-1,4,5-triphosphate
 D-myo-inositol-1,4,5-triphosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -27.61 | ± | kJ/mol |
| ΔH | -46.86 | ± | kJ/mol |
| -TΔS | -19.25 | ± | kJ/mol |
| KD | 24.0 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
The estimated error in Kd upon repeated measurements approximately 25% (standard error of the mean) |
General comments | |
| Warnings |
DeltaG (-27.61) != -R.T.ln(Ka) (-26.37). Tolerance range from -27.89 to -27.34 |
||
| Instrument | Not stated | ||
| Temp. (K) | 298.15 | pH | 7.0 |
| Buffer | Sodium Chloride 150.00 mM PIPES 20.00 mM DTT 0.20 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2004] 43, 10979-10987 | ||