ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Wheat Germ Agglutinin 1 | ||
| Ligand name |
Chitobiose
 Chitobiose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 2.0 | ± | |
| ΔG | -20.5 | ± | kJ/mol |
| ΔH | -112.55 | ± | kJ/mol |
| -TΔS | -92.05 | ± | kJ/mol |
| KD | 238.1 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Ka and delta H were obtainied from a non-linear regression fit to the isotherm and the error of these values were always less than 7% (n=4) and less than 4% (n=2). Where n is the assumed number of biniding sites. |
General comments | |
| Warnings |
DeltaG (-20.50) != -R.T.ln(Ka) (-20.75). Tolerance range from -20.71 to -20.30 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 299.15 | pH | 7.0 |
| Buffer | Sodium Phosphate 50.00 mM Potassium Chloride 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochim Biophys Acta. [2002] 1569, 10-20 | ||