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Structure-Calorimetry Of Reported Protein Interactions Online

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ITC

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ITC datum

Protein and Ligand
Protein name	Wheat Germ Agglutinin 1
Ligand name	Gal(1)-(6)Nag Gal(1)-(6)Nag
Interaction type	Biological
ITC data	ITC data
Structure data	Structure data
ITC Information
n	2.0	±
ΔG	-16.32	±	kJ/mol
ΔH	-63.18	±	kJ/mol
-TΔS	-46.86	±	kJ/mol
KD	1724.14	±	uM
ΔCP		±
Notes on error determination	Ka and delta H were obtainied from a non-linear regression fit to the isotherm and the error of these values were always less than 7% (n=4) and less than 4% (n=2). Where n is the assumed number of biniding sites.
General comments
Warnings	DeltaG (-16.32) != -R.T.ln(Ka) (-15.83). Tolerance range from -16.48 to -16.15 Affinity (1724.1379) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-16.32) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow.
Instrument	Microcal MCS Titration Calorimeter
Temp. (K)	299.15	pH	7.0
Buffer	Sodium Phosphate 50.00 mM Potassium Chloride 50.00 mM
Cell content	Protein
Reference	Biochim Biophys Acta. [2002] 1569, 10-20

Disclaimer

This is an ongoing project and, although SCORPIO has been curated with great care, it may contain errors. If you spot an error please contact us. We take no responsibility for the data within SCORPIO, nor any responsibility for the consequences of using the data within SCORPIO. No contract, warranty or liability is implied by using the data within SCORPIO.

If you make use of SCORPIO please cite J. Mol. Biol. [2008] 384, 1002-1017
Tjelvar S. G. Olsson, Mark A. Williams, Will R. Pitt and John E. Ladbury