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ITC datum

Protein and Ligand
Protein name Wheat Germ Agglutinin 2
Ligand name Nag(1)-(6)gal Nag(1)-(6)gal diagram
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n 4.0 ±  
ΔG -23.01 ± kJ/mol
ΔH -46.86 ± kJ/mol
-TΔS -23.85 ± kJ/mol
KD 90.09 ± uM
Notes on error determination Ka and delta H were obtainied from a non-linear regression fit to the isotherm and the error of these values were always less than 7% (n=4) and less than 4% (n=2). Where n is the assumed number of biniding sites.
General comments
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 299.15 pH 7.0
Buffer Sodium Phosphate 50.00 mM Potassium Chloride 50.00 mM
Cell content Protein
Reference Biochim Biophys Acta. [2002] 1569, 10-20