ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Polyandrocarpa Lectin | ||
| Ligand name |
Gal(1)-(6)gal
 Gal(1)-(6)gal |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -19.5 | ± | kJ/mol |
| ΔH | -46.8 | ± | kJ/mol |
| -TΔS | -27.28 | ± | kJ/mol |
| KD | 380.0 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
Affinity (380.0000) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-19.50) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 297.85 | pH | 7.8 |
| Buffer | MOPS 5.00 mM Calcium Chloride 5.00 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [1999] 290, 867-879 | ||