ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Purine Nucleoside Phosphorylase | ||
| Ligand name |
Guanosine
 Guanosine |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.51 | ± | |
| ΔG | -23.56 | ± | kJ/mol |
| ΔH | -99.16 | ± | kJ/mol |
| -TΔS | -75.6 | ± | kJ/mol |
| KD | 80.0 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
Typo in figure 8: enthalpy unit corrected to cal/mol from kcal/mol. Deduced from the Ka and the ΔS.
Authors contacted. |
||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 7.0 |
| Buffer | Potassium Acetate 20.00 mM Ammonium Sulfate 0.10 mM Magnesium Sulfate 0.10 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2004] 337, 1149-1160 | ||