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ITC datum

Protein and Ligand
Protein name Purine Nucleoside Phosphorylase
Ligand name Guanosine Guanosine diagram
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n 0.51 ±  
ΔG -23.56 ± kJ/mol
ΔH -99.16 ± kJ/mol
-TΔS -75.6 ± kJ/mol
KD 80.0 ± uM
Notes on error determination Not applicable
General comments
Warnings Typo in figure 8: enthalpy unit corrected to cal/mol from kcal/mol. Deduced from the Ka and the ΔS. Authors contacted.
Instrument VP-ITC Microcalorimeter
Temp. (K) 298.15 pH 7.0
Buffer Potassium Acetate 20.00 mM Ammonium Sulfate 0.10 mM Magnesium Sulfate 0.10 mM
Cell content Protein
Reference J Mol Biol. [2004] 337, 1149-1160