ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Nucleoside Diphosphate Kinase (variant 1) | ||
| Ligand name |
Adenosine-5'-diphosphate
 Adenosine-5'-diphosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 5.9 | ± 0.4 | |
| ΔG | -22.05 | ± 0.42 | kJ/mol |
| ΔH | -17.57 | ± 2.51 | kJ/mol |
| -TΔS | 4.6 | ± 2.51 | kJ/mol |
| KD | 117.1 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Mean value of three independent measurements +- s.e. |
General comments | |
| Warnings | |||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 293.15 | pH | 8.0 |
| Buffer | HEPES 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2001] 40, 4583-4589 | ||