ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Nucleoside Diphosphate Kinase (variant 1) | ||
| Ligand name |
Guanosine-5'-diphosphate
 Guanosine-5'-diphosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 4.9 | ± | |
| ΔG | -24.02 | ± | kJ/mol |
| ΔH | -23.01 | ± | kJ/mol |
| -TΔS | 1.26 | ± | kJ/mol |
| KD | 52.08 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaG (-24.02) != DeltaH (-23.01) - T.DeltaS (1.26) (=-24.27). Tolerance range from -24.26 to -23.78 |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 293.15 | pH | 8.0 |
| Buffer | HEPES 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2001] 40, 4583-4589 | ||