ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Major Urinary Protein I | ||
| Ligand name |
2-(methyl)-1,4-dihydrothiazole
 2-(methyl)-1,4-dihydrothiazole |
||
| Interaction type | Misc | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.06 | ± | |
| ΔG | -23.22 | ± | kJ/mol |
| ΔH | -43.1 | ± | kJ/mol |
| -TΔS | -20.31 | ± | kJ/mol |
| KD | 101.0 | ± | uM |
| ΔCP | -0.56 | ± 0.52 | kJ/mol/K |
| Notes on error determination |
SBT binding was measured 3 times at 25C, other values are from single measurements. For most ligands, fitted errors in Kd, n, DH, DS, and DG values are less than or equal to 5, 2, 3, 5 and 5% respectively. For MT they are as high as 9, 11, 14, 30 and 11% |
General comments | |
| Warnings |
DeltaG (-23.22) != DeltaH (-43.10) - T.DeltaS (-20.31) (=-22.78). Tolerance range from -23.45 to -22.99 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 303.45 | pH | 6.3 |
| Buffer | Phosphate 10.00 mM Sodium Azide 0.02 % | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2003] 42, 6302-6309 | ||