ITC

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ITC datum

Protein and Ligand
Protein name Serum Albumin
Ligand name 2-bromo-2-chloro-1,1,1-trifluoroethane 2-bromo-2-chloro-1,1,1-trifluoroethane diagram
2-bromo-2-chloro-1,1,1-trifluoroethane
Interaction type Misc
ITC data ITC data
Structure data Structure data
ITC Information
n 7.6 ± 5.7  
ΔG -15.7 ± kJ/mol
ΔH -5.52 ± 4.31 kJ/mol
-TΔS 10.18 ± kJ/mol
KD 1631.32 ± 11363.64 uM
ΔCP ±
Notes on error determination Larger deviation estimates were obsereved for the halothane-HSA interaction. Because the number of sites for halothane has been reported as seven the data was refitted by fixing n = 7.
General comments
Warnings Affinity (1631.3214) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow.
DeltaG (-15.70) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow.
DeltaH (-5.52) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0.
Instrument VP-ITC Microcalorimeter
Temp. (K) 293.15 pH 7.0
Buffer Sodium Phosphate 20.00 mM Sodium Chloride 130.00 mM
Cell content Protein
Reference Biochem. J. [2004] 380, 147-152