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ITC datum

Protein and Ligand
Protein name HIV-1 Protease (Q7K/L33I/I50L/L63I/A71V)
Ligand name Ritonavir Ritonavir diagram
Ritonavir
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n 5.0 ±  
ΔG -62.76 ± kJ/mol
ΔH -33.47 ± kJ/mol
-TΔS 29.29 ± kJ/mol
KD 0.0 ± uM
ΔCP ±
Notes on error determination In order to measure Kd values for tight binding inhibitors, acetyl pepstatin was used as a competition displacement ligand. Errors determined using non-linear least-squares analysis. Kd, G and -T.S represent upper limit values.
General comments Binding too tight to measure directly. Data obtained by competition ITC.
Warnings Affinity (0.0000) less than 0.0000. It is difficult to interpred ITC data if Affinity is less than 0.0000 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments.
DeltaG (-62.76) less than -60.00. It is difficult to interpred ITC data if DeltaG is less than -60.00 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments.
Instrument VP-ITC Microcalorimeter
Temp. (K) 298.15 pH 5.0
Buffer DMSO 2.00 % Sodium Acetate 10.00 mM
Cell content Protein
Reference Antimicrob. Agents Chemother. [2005] 49, 3825-3832