ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | HIV-1 Protease (Q7K/L33I/I50L/L63I/A71V) | ||
| Ligand name |
Nelfinavir mesylate ag1343
 Nelfinavir mesylate ag1343 |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 5.0 | ± | |
| ΔG | -55.86 | ± 0.5 | kJ/mol |
| ΔH | -5.02 | ± | kJ/mol |
| -TΔS | 51.04 | ± | kJ/mol |
| KD | 0.0 | ± 0.0 | uM |
| ΔCP | ± | ||
| Notes on error determination |
In order to measure Kd values for tight binding inhibitors, acetyl pepstatin was used as a competition displacement ligand. Errors determined using non-linear least-squares analysis. |
General comments |
Binding too tight to measure directly.
Data obtained by competition ITC. |
| Warnings |
DeltaH (-5.02) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. |
||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 5.0 |
| Buffer | DMSO 2.00 % Sodium Acetate 10.00 mM | ||
| Cell content | Protein | ||
| Reference | Antimicrob. Agents Chemother. [2005] 49, 3825-3832 | ||