ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Nucleoside Diphosphate Kinase (variant 1) | ||
| Ligand name |
Adenosine-5'-diphosphate
 Adenosine-5'-diphosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 5.4 | ± | |
| ΔG | -24.27 | ± | kJ/mol |
| ΔH | -10.46 | ± | kJ/mol |
| -TΔS | 13.81 | ± | kJ/mol |
| KD | 45.66 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings | |||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 293.15 | pH | 8.0 |
| Buffer | HEPES 50.00 mM Magnesium Chloride Standard | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2001] 40, 4583-4589 | ||