ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | Dihydrodipicolinate Reductase | ||
Ligand name |
3'-dihydro-nicotinamide-adenine-dinucleotide phosphate
3'-dihydro-nicotinamide-adenine-dinucleotide phosphate |
||
Interaction type | Biological | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | 0.99 | ± 0.02 | |
ΔG | -30.5 | ± 0.29 | kJ/mol |
ΔH | -50.96 | ± 1.21 | kJ/mol |
-TΔS | -20.54 | ± 0.93 | kJ/mol |
KD | 5.05 | ± 0.56 | uM |
ΔCP | ± | ||
Notes on error determination |
Errors reported were propagated from the errors of Ka and H, respectively. |
General comments | |
Warnings | |||
Instrument | Omega ITC Microcalorimeter | ||
Temp. (K) | 301.15 | pH | 7.8 |
Buffer | HEPES/KOH 20.00 mM | ||
Cell content | Protein | ||
Reference | Biochemistry [1996] 35, 13294-13302 |