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ITC datum

Protein and Ligand
Protein name Fatty Acid-Binding Protein, Brain
Ligand name Lauric acid Lauric acid diagram
Lauric acid
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n 1.0 ±  
ΔG -36.9 ± kJ/mol
ΔH -37.3 ± 1.8 kJ/mol
-TΔS -0.4 ± 2.2 kJ/mol
KD 0.44 ± 0.06 uM
ΔCP ±
Notes on error determination Data were fitted to a model assuming one binding site. (Mean +/- S.D. n=3).
General comments
Warnings
Instrument Omega ITC Microcalorimeter
Temp. (K) 303.15 pH 8.0
Buffer Tris HCl 50.00 mM Sodium Azide 0.05 % EtSH 1.00 mM
Cell content Protein
Reference J. Biol. Chem [2000] 275, 27045-27154