ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Fatty Acid-Binding Protein, Brain | ||
| Ligand name |
Arachidonic acid
 Arachidonic acid |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.0 | ± | |
| ΔG | -38.7 | ± | kJ/mol |
| ΔH | -60.3 | ± 6.6 | kJ/mol |
| -TΔS | -21.6 | ± 6.6 | kJ/mol |
| KD | 207.0 | ± 19.0 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Data were fitted to a model assuming one binding site. (Mean +/- S.D. n=3). |
General comments | |
| Warnings |
DeltaG (-38.70) != -R.T.ln(Ka) (-21.38). Tolerance range from -39.09 to -38.31 |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 303.15 | pH | 8.0 |
| Buffer | Tris HCl 50.00 mM Sodium Azide 0.05 % EtSH 1.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Biol. Chem [2000] 275, 27045-27154 | ||