ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Fatty Acid-Binding Protein, Brain | ||
| Ligand name |
5,8,11,14,17-eicosapentaenoic acid
 5,8,11,14,17-eicosapentaenoic acid |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.0 | ± | |
| ΔG | -42.7 | ± | kJ/mol |
| ΔH | -52.2 | ± 2.4 | kJ/mol |
| -TΔS | -9.5 | ± 3.3 | kJ/mol |
| KD | 48.1 | ± 21.0 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Data were fitted to a model assuming one binding site. (Mean +/- S.D. n=3). |
General comments | |
| Warnings |
DeltaG (-42.70) != -R.T.ln(Ka) (-25.06). Tolerance range from -43.13 to -42.27 |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 303.15 | pH | 8.0 |
| Buffer | Tris HCl 50.00 mM Sodium Azide 0.05 % EtSH 1.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Biol. Chem [2000] 275, 27045-27154 | ||