ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Erythrina Crista-Galli Lectin | ||
| Ligand name |
Lactose
 Lactose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -19.9 | ± 0.1 | kJ/mol |
| ΔH | -29.9 | ± 1.4 | kJ/mol |
| -TΔS | -10.02 | ± -0.42 | kJ/mol |
| KD | 322.58 | ± 10000.0 | uM |
| ΔCP | ± | ||
| Notes on error determination |
The uncertainties reported for Kb and Hb are the standard deviations from the average value, determined from at least two different titrations. |
General comments | |
| Warnings |
Affinity (322.5806) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-19.90) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow. |
||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 7.4 |
| Buffer | Sodium Phosphate 20.00 mM Sodium Chloride 0.15 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2002] 321, 69-83 | ||