ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Erythrina Crista-Galli Lectin | ||
| Ligand name |
2'-alpha-l-fucosyllactose
 2'-alpha-l-fucosyllactose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -20.0 | ± 0.1 | kJ/mol |
| ΔH | -19.7 | ± 1.1 | kJ/mol |
| -TΔS | 0.3 | ± | kJ/mol |
| KD | 312.5 | ± 5000.0 | uM |
| ΔCP | ± | ||
| Notes on error determination |
The uncertainties reported for Kb and Hb are the standard deviations from the average value, determined from at least two different titrations. |
General comments | |
| Warnings | |||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 7.4 |
| Buffer | Sodium Phosphate 20.00 mM Sodium Chloride 0.15 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2002] 321, 69-83 | ||