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ITC datum

Protein and Ligand
Protein name HIV-1 Protease (Q7K/K14R/V82T/I84V)
Ligand name Amprenavir Amprenavir diagram
Amprenavir
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -43.93 ± kJ/mol
ΔH -16.32 ± kJ/mol
-TΔS 27.61 ± kJ/mol
KD 0.02 ± uM
ΔCP ±
Notes on error determination Not applicable
General comments Binding too tight to measure directly. Data obtained by competition ITC.
Warnings DeltaG (-43.93) != -R.T.ln(Ka) (-43.11). Tolerance range from -44.37 to -43.49
Instrument VP-ITC Microcalorimeter
Temp. (K) 293.15 pH 5.0
Buffer DMSO 2.00 % Sodium Acetate 10.00 mM Tris(2-carboxyethyl)phosphine (TCEP) 2.00 mM
Cell content Protein
Reference J. Med. Chem. [2005] 48, 1813-1822