ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | HIV-1 Protease (Q7K/K14R/V82T/I84V) | ||
Ligand name |
Amprenavir
Amprenavir |
||
Interaction type | Synthetic ligands | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | ± | ||
ΔG | -43.93 | ± | kJ/mol |
ΔH | -16.32 | ± | kJ/mol |
-TΔS | 27.61 | ± | kJ/mol |
KD | 0.02 | ± | uM |
ΔCP | ± | ||
Notes on error determination |
Not applicable |
General comments |
Binding too tight to measure directly.
Data obtained by competition ITC. |
Warnings |
DeltaG (-43.93) != -R.T.ln(Ka) (-43.11). Tolerance range from -44.37 to -43.49 |
||
Instrument | VP-ITC Microcalorimeter | ||
Temp. (K) | 293.15 | pH | 5.0 |
Buffer | DMSO 2.00 % Sodium Acetate 10.00 mM Tris(2-carboxyethyl)phosphine (TCEP) 2.00 mM | ||
Cell content | Protein | ||
Reference | J. Med. Chem. [2005] 48, 1813-1822 |