ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | HIV-1 Protease (Q7K/K14R/V82T/I84V) | ||
| Ligand name |
Tmc126
 Tmc126 |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -58.99 | ± | kJ/mol |
| ΔH | -40.17 | ± | kJ/mol |
| -TΔS | 18.83 | ± | kJ/mol |
| KD | 0.0 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments |
Binding too tight to measure directly.
Data obtained by competition ITC. |
| Warnings |
DeltaG (-58.99) != -R.T.ln(Ka) (-58.15). Tolerance range from -59.58 to -58.40 |
||
| Instrument | VP-ITC Microcalorimeter | ||
| Temp. (K) | 293.15 | pH | 5.0 |
| Buffer | DMSO 2.00 % Sodium Acetate 10.00 mM Tris(2-carboxyethyl)phosphine (TCEP) 2.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Med. Chem. [2005] 48, 1813-1822 | ||