ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Endo-1,4-Beta Glucanase EngF | ||
| Ligand name |
Cellotetraose
 Cellotetraose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -17.53 | ± 0.07 | kJ/mol |
| ΔH | -74.59 | ± 2.3 | kJ/mol |
| -TΔS | -57.06 | ± | kJ/mol |
| KD | 833.33 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Errors represent standard deviations of three experiments. |
General comments | |
| Warnings |
Affinity (833.3333) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-17.53) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow. |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 297.37 | pH | 7.0 |
| Buffer | Potassium Phosphate Buffer 50.00 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2001] 314, 797-806 | ||