ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Endo-1,4-Beta Glucanase EngF (D54A) | ||
| Ligand name |
Cellohexaose
 Cellohexaose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -22.02 | ± 0.45 | kJ/mol |
| ΔH | -51.63 | ± 0.22 | kJ/mol |
| -TΔS | -29.61 | ± | kJ/mol |
| KD | 138.89 | ± 5000.0 | uM |
| ΔCP | -0.53 | ± 0.03 | kJ/mol/K |
| Notes on error determination |
Errors represent standard deviations of three experiments. |
General comments | |
| Warnings | |||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.18 | pH | 7.0 |
| Buffer | Potassium Phosphate Buffer 50.00 mM | ||
| Cell content | Protein | ||
| Reference | J Mol Biol. [2001] 314, 797-806 | ||