ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Nucleoside Diphosphate Kinase (variant 1) | ||
| Ligand name |
Phosphoaminophosphonic acid-adenylate ester
 Phosphoaminophosphonic acid-adenylate ester |
||
| Interaction type | Misc | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 5.6 | ± | |
| ΔG | -20.33 | ± | kJ/mol |
| ΔH | -2.51 | ± | kJ/mol |
| -TΔS | 17.99 | ± | kJ/mol |
| KD | 238.1 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaH (-2.51) in range 10.00 to -10.00. It is difficult to interpret ITC data as DeltaH approaches 0. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 293.15 | pH | 8.0 |
| Buffer | HEPES 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2001] 40, 4583-4589 | ||