ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Streptavidin (synthetic construct) | ||
| Ligand name |
HDHPQNL
 HDHPQNL |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.96 | ± 0.02 | |
| ΔG | -20.25 | ± 0.13 | kJ/mol |
| ΔH | -79.5 | ± 2.68 | kJ/mol |
| -TΔS | -59.41 | ± | kJ/mol |
| KD | 282.33 | ± 6849.32 | uM |
| ΔCP | ± | ||
| Notes on error determination |
Data are reported as the mean fro two separate titrations with +/- 1 standard deviation in parentheses. |
General comments | |
| Warnings | |||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 7.62 |
| Buffer | Potassium Phosphate 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [1992] 31, 9350-9354 | ||