ITC

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ITC datum

Protein and Ligand
Protein name Streptavidin
Ligand name Biotin Biotin diagram
Biotin
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -76.57 ± kJ/mol
ΔH -104.18 ± 1.67 kJ/mol
-TΔS -27.61 ± kJ/mol
KD 0.0 ± uM
ΔCP -1.44 ± 0.05 kJ/mol/K
Notes on error determination Not available
General comments
Warnings Corrected typo in original paper: table 1A, row 1, col 2 changed to -18.3 from -18. Deduced from ΔG, TΔS, and the Ka. Authors contacted.
Affinity (0.0000) less than 0.0000. It is difficult to interpred ITC data if Affinity is less than 0.0000 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments.
DeltaG (-76.57) less than -60.00. It is difficult to interpred ITC data if DeltaG is less than -60.00 as the sigmoidal curve becomes too sharp. This can be overcome by performing displacement experiments.
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.51 pH 7.75
Buffer Unkown Standard
Cell content Protein
Reference Protein Sci. [1998] 7, 848-859