ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Nucleoside Diphosphate Kinase (variant 1) | ||
| Ligand name |
Thymidine-5'-diphosphate
 Thymidine-5'-diphosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 9.2 | ± 0.3 | |
| ΔG | -19.58 | ± 0.42 | kJ/mol |
| ΔH | -13.39 | ± 0.84 | kJ/mol |
| -TΔS | 6.28 | ± 1.26 | kJ/mol |
| KD | 322.58 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Mean value of three independent measurements +- s.e. |
General comments | |
| Warnings |
Affinity (322.5806) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow. DeltaG (-19.58) greater than -20.00. It is difficult to interpred ITC data if DeltaG is greater than -20.00 as the sigmoidal curve becomes too shallow. |
||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 293.15 | pH | 8.0 |
| Buffer | HEPES 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2001] 40, 4583-4589 | ||