ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Heat Shock Protein 90 N-terminal Domain, (E. coli expressed) | ||
| Ligand name |
Geldanamycin
 Geldanamycin |
||
| Interaction type | Synthetic ligands | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | ± | ||
| ΔG | -44.1 | ± | kJ/mol |
| ΔH | -33.7 | ± | kJ/mol |
| -TΔS | 0.03 | ± | kJ/mol |
| KD | 0.02 | ± | uM |
| ΔCP | ± | ||
| Notes on error determination |
Not applicable |
General comments | |
| Warnings |
DeltaG (-44.10) != DeltaH (-33.70) - T.DeltaS (0.03) (=-33.73). Tolerance range from -44.54 to -43.66 |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 7.5 |
| Buffer | EDTA 1.00 mM Tris HCl 20.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Med. Chem. [1999] 42, 260-266 | ||