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ITC datum

Protein and Ligand
Protein name Heat Shock Protein 90 N-terminal Domain, (E. coli expressed)
Ligand name Radicol Radicol diagram
Interaction type Synthetic ligands
ITC data ITC data
Structure data Structure data
ITC Information
n ±  
ΔG -48.9 ± kJ/mol
ΔH -22.8 ± kJ/mol
-TΔS 26.09 ± kJ/mol
KD 0.0 ± uM
Notes on error determination Not applicable
General comments ITC binding curves at this high affinity become too steep for accurate fitting, and the estimation of contingent parameters isconsequently less reliable.
Entropy units reported should be J/mol/K
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.15 pH 7.5
Buffer EDTA 1.00 mM Tris HCl 20.00 mM
Cell content Protein
Reference J. Med. Chem. [1999] 42, 260-266