ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | Endo-1,4-Beta-Xylanase A, C2 Domain (Residues 871-1059) | ||
Ligand name |
Cellotetraose
Cellotetraose |
||
Interaction type | Biological | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | 0.88 | ± 0.02 | |
ΔG | -32.85 | ± 0.09 | kJ/mol |
ΔH | -45.26 | ± 0.33 | kJ/mol |
-TΔS | -12.41 | ± -0.34 | kJ/mol |
KD | 1.75 | ± 50.0 | uM |
ΔCP | -0.17 | ± 0.01 | kJ/mol/K |
Notes on error determination |
Errors represent the standard deviations of triplicate or quadruplicate experiments or propagation of error. |
General comments | |
Warnings | |||
Instrument | Microcal MCS Titration Calorimeter | ||
Temp. (K) | 298.15 | pH | 7.0 |
Buffer | Phosphate Buffer 50.00 mM | ||
Cell content | Protein | ||
Reference | Biochemistry [2001] 40, 6240-6247 |