ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Endo-1,4-Beta-Xylanase A, C2 Domain (Residues 871-1059) | ||
| Ligand name |
Lactose
 Lactose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.89 | ± 0.01 | |
| ΔG | -32.72 | ± 0.09 | kJ/mol |
| ΔH | -43.75 | ± 0.12 | kJ/mol |
| -TΔS | -11.03 | ± -0.15 | kJ/mol |
| KD | 1.86 | ± 52.63 | uM |
| ΔCP | -0.28 | ± 0.03 | kJ/mol/K |
| Notes on error determination |
Errors represent the standard deviations of triplicate or quadruplicate experiments or propagation of error. |
General comments | |
| Warnings | |||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 7.0 |
| Buffer | Phosphate Buffer 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2001] 40, 6240-6247 | ||