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ITC datum

Protein and Ligand
Protein name Endo-1,4-Beta-Xylanase A, C2 Domain (Residues 871-1059)
Ligand name Glucose Glucose diagram
Glucose
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n 0.75 ±  
ΔG -20.19 ± kJ/mol
ΔH -31.18 ± 0.53 kJ/mol
-TΔS -11.37 ± kJ/mol
KD 333.33 ± uM
ΔCP -0.14 ± 0.07 kJ/mol/K
Notes on error determination Errors represent the standard deviations of triplicate or quadruplicate experiments or propagation of error.
General comments
Warnings Ambigious error: ΔH - TΔS=-19.81, not equal to ΔG=-20.19 and -R.T.ln(Ka)=-19.846336848312, not equal to ΔG=-20.19
DeltaG (-20.19) != -R.T.ln(Ka) (-19.85). Tolerance range from -20.39 to -19.99
DeltaG (-20.19) != DeltaH (-31.18) - T.DeltaS (-11.37) (=-19.81). Tolerance range from -20.39 to -19.99
Affinity (333.3333) greater than 313.4408. It is difficult to interpred ITC data if Affinity is greater than 313.4408 as the sigmoidal curve becomes too shallow.
Instrument Microcal MCS Titration Calorimeter
Temp. (K) 298.15 pH 7.0
Buffer Phosphate Buffer 50.00 mM
Cell content Protein
Reference Biochemistry [2001] 40, 6240-6247