ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Endo-1,4-Beta-Xylanase A, C2 Domain (Residues 871-1059) | ||
| Ligand name |
Maltose
 Maltose |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.91 | ± 0.01 | |
| ΔG | -30.38 | ± 0.03 | kJ/mol |
| ΔH | -39.75 | ± 0.06 | kJ/mol |
| -TΔS | -9.37 | ± | kJ/mol |
| KD | 4.76 | ± 500.0 | uM |
| ΔCP | -0.3 | ± 0.01 | kJ/mol/K |
| Notes on error determination |
Errors represent the standard deviations of triplicate or quadruplicate experiments or propagation of error. |
General comments | |
| Warnings |
ΔG changed to -30.38 from -30.39, derived from affinity (-R.T.ln(Ka)=-30.378), the ΔH and ΔS(ΔH-TΔS=-30.38) |
||
| Instrument | Microcal MCS Titration Calorimeter | ||
| Temp. (K) | 298.15 | pH | 7.0 |
| Buffer | Phosphate Buffer 50.00 mM | ||
| Cell content | Protein | ||
| Reference | Biochemistry [2001] 40, 6240-6247 | ||