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ITC datum

Protein and Ligand
Protein name Ribonuclease T1
Ligand name Guanosine-3'-monophosphate Guanosine-3'-monophosphate diagram
Guanosine-3'-monophosphate
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n 1.0 ± 0.02  
ΔG -28.12 ± kJ/mol
ΔH -31.97 ± 0.08 kJ/mol
-TΔS -3.85 ± kJ/mol
KD 11.85 ± 625.0 uM
ΔCP -0.22 ± 0.04 kJ/mol/K
Notes on error determination Uncertainties represent standard errors of the mean.
General comments Number of titrations: 3.
Warnings
Instrument Omega ITC Microcalorimeter
Temp. (K) 298.15 pH 5.5
Buffer Sodium Acetate 50.00 mM
Cell content Protein
Reference J. Phys. Chem. [1992] 96, 4052-4056