ITC

Browse


ISMB logo
Biochemical Journal logo

ITC datum

Protein and Ligand
Protein name Ribonuclease T1
Ligand name Guanosine-3'-monophosphate Guanosine-3'-monophosphate diagram
Guanosine-3'-monophosphate
Interaction type Biological
ITC data ITC data
Structure data Structure data
ITC Information
n 1.08 ± 0.05  
ΔG -27.96 ± kJ/mol
ΔH -35.65 ± 0.17 kJ/mol
-TΔS -7.69 ± kJ/mol
KD 18.25 ± 2500.0 uM
ΔCP -0.22 ± 0.04 kJ/mol/K
Notes on error determination Uncertainties represent standard errors of the mean.
General comments Number of titrations: 3.
Warnings
Instrument Omega ITC Microcalorimeter
Temp. (K) 308.15 pH 5.5
Buffer Sodium Acetate 50.00 mM
Cell content Protein
Reference J. Phys. Chem. [1992] 96, 4052-4056