ITC datum
Protein and Ligand | |||
---|---|---|---|
Protein name | Ribonuclease T1 | ||
Ligand name |
Guanosine-3'-monophosphate
Guanosine-3'-monophosphate |
||
Interaction type | Biological | ||
ITC data | ITC data | ||
Structure data | Structure data | ||
ITC Information | |||
n | 1.08 | ± 0.05 | |
ΔG | -27.96 | ± | kJ/mol |
ΔH | -35.65 | ± 0.17 | kJ/mol |
-TΔS | -7.69 | ± | kJ/mol |
KD | 18.25 | ± 2500.0 | uM |
ΔCP | -0.22 | ± 0.04 | kJ/mol/K |
Notes on error determination |
Uncertainties represent standard errors of the mean. |
General comments |
Number of titrations: 3. |
Warnings | |||
Instrument | Omega ITC Microcalorimeter | ||
Temp. (K) | 308.15 | pH | 5.5 |
Buffer | Sodium Acetate 50.00 mM | ||
Cell content | Protein | ||
Reference | J. Phys. Chem. [1992] 96, 4052-4056 |