ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Ribonuclease T1 | ||
| Ligand name |
Guanosine-5'-monophosphate
 Guanosine-5'-monophosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.1 | ± 0.01 | |
| ΔG | -22.74 | ± | kJ/mol |
| ΔH | -35.23 | ± 0.67 | kJ/mol |
| -TΔS | -12.49 | ± | kJ/mol |
| KD | 103.84 | ± 3030.3 | uM |
| ΔCP | -0.26 | ± 0.03 | kJ/mol/K |
| Notes on error determination |
Uncertainties represent standard errors of the mean. |
General comments |
Number of titrations: 4. |
| Warnings | |||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 5.5 |
| Buffer | Sodium Acetate 50.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Phys. Chem. [1992] 96, 4052-4056 | ||