ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Ribonuclease T1 | ||
| Ligand name |
Guanosine-5'-monophosphate
 Guanosine-5'-monophosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 1.12 | ± 0.02 | |
| ΔG | -22.57 | ± | kJ/mol |
| ΔH | -38.91 | ± 0.54 | kJ/mol |
| -TΔS | -16.34 | ± | kJ/mol |
| KD | 149.48 | ± 8333.33 | uM |
| ΔCP | -0.26 | ± 0.03 | kJ/mol/K |
| Notes on error determination |
Uncertainties represent standard errors of the mean. |
General comments |
Number of titrations: 11. |
| Warnings | |||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 308.15 | pH | 5.5 |
| Buffer | Sodium Acetate 50.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Phys. Chem. [1992] 96, 4052-4056 | ||