ITC datum
| Protein and Ligand | |||
|---|---|---|---|
| Protein name | Ribonuclease T1 | ||
| Ligand name |
Adenosine-2'-monophosphate
 Adenosine-2'-monophosphate |
||
| Interaction type | Biological | ||
| ITC data | ITC data | ||
| Structure data | Structure data | ||
| ITC Information | |||
| n | 0.96 | ± 0.04 | |
| ΔG | -21.02 | ± | kJ/mol |
| ΔH | -35.9 | ± 1.13 | kJ/mol |
| -TΔS | -14.88 | ± | kJ/mol |
| KD | 207.47 | ± 5263.16 | uM |
| ΔCP | -0.21 | ± 0.01 | kJ/mol/K |
| Notes on error determination |
Uncertainties represent standard errors of the mean. |
General comments |
Number of titrations: 3. |
| Warnings | |||
| Instrument | Omega ITC Microcalorimeter | ||
| Temp. (K) | 298.15 | pH | 5.5 |
| Buffer | Sodium Acetate 50.00 mM | ||
| Cell content | Protein | ||
| Reference | J. Phys. Chem. [1992] 96, 4052-4056 | ||